Journal: Molecular Metabolism
Article Title: Membrane-associated ring–CH–type finger 2 protects against metabolic dysfunction-associated fatty liver disease by targeting fatty acid synthase
doi: 10.1016/j.molmet.2025.102137
Figure Lengend Snippet: Overexpression of hepatic MARCH2 ameliorates MAFLD in ob/ob mice. (A) The rAAV8 with TBG promoter-driven MARCH2 (AAV-MARCH2) or GFP (AAV-GFP) were delivered by tail vein injection to 8-week-old ob/ob mice and fed with a chow diet for another 7 weeks. This schema diagram was created by Figdraw (authorization code: TTPIO86bdd). Body weight (B), morphology of the mice and liver (C), GTT (D), ITT (E), area under curve(AUC) of GTT(F), AUC of ITT(G), liver weight (H), ratios of liver weight to body weight (I), liver HE staining (J), liver Oil Red O staining (K), liver TG contents (L), liver MARCH2 mRNA expression (M), and FASN protein levels (N) of AAV-MARCH2 and AAV-GFP mice were detected. n = 6–8 mice per group, and the data were expressed as Mean ± SEM. Two-tailed unpaired student’s t -test were applied.
Article Snippet: The rAAV8 with thyroxine binding globulin (TBG) promoter-driven MARCH2 coding sequence (AAV-MARCH2) or GFP control (AAV-GFP) were generated by GenePharma (Shanghai, China).
Techniques: Over Expression, Injection, Staining, Expressing, Two Tailed Test